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Item #:
223862062
TAE Buffer 1X, Tris Acetate-EDTA Buffer, Ready-to-Use DNA Electrophoresis Running Buffer, pH 8.00.1, Agarose Gel Buffer for Molecular Biology
Item #:
223862062
SBD 215
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Product Details
| Item Weight | 1.1 lbs (500 grams) |
Product Description
TAE Buffer 1X, Tris Acetate-EDTA Buffer, Ready-to-Use DNA Electrophoresis Running Buffer, pH 8.00.1, Agarose Gel Buffer for Molecular Biology Research, 500mL
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Customer Questions & Answers
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Question:
What is Tris Acetate-EDTA Buffer used for?
Answer: Tris Acetate-EDTA Buffer, commonly referred to as TAE buffer, is primarily used in DNA electrophoresis as a medium for the separation of DNA fragments. It maintains a stable pH and provides essential ions that facilitate the migration of nucleic acids during electrophoresis. This buffer allows researchers to analyze DNA samples effectively, making it ideal for applications such as gel electrophoresis, cloning, and DNA purification. -
Question:
What is the pH of the Tris Acetate-EDTA Buffer?
Answer: The Tris Acetate-EDTA Buffer is specifically formulated to have a pH of 8.0, which is optimal for DNA stability and migration. This pH level is essential, as it ensures that the nucleic acids remain in their charged state during electrophoresis, allowing them to move towards the positive electrode effectively. By maintaining this pH, researchers can expect consistent and reproducible results in their experiments. -
Question:
How do you prepare TAE buffer for DNA electrophoresis?
Answer: To prepare TAE buffer for DNA electrophoresis, simply dilute the concentrated stock solution in deionized water to achieve the desired working concentration, typically 1×. Ensure the final volume is sufficient for your gel and electrophoresis apparatus. It's crucial to mix the solution thoroughly and check the pH with a pH meter to confirm it stays at 8.0, which is optimal for effective DNA separation. -
Question:
Can I reuse TAE buffer after electrophoresis?
Answer: Yes, TAE buffer can be reused for electrophoresis, especially if it remains clear and free from contamination. However, repeated use may lead to decreased effectiveness due to the accumulation of DNA, proteins, and other impurities. It's recommended to monitor the buffer's clarity and performance; if the buffer becomes discolored or shows reduced efficiency, prepare a fresh batch to ensure optimal results in your experiments. -
Question:
How should I store Tris Acetate-EDTA Buffer?
Answer: Tris Acetate-EDTA Buffer should be stored at room temperature in a tightly sealed container to prevent contamination and evaporation. Keeping it away from direct sunlight and extreme temperatures is essential to maintain its stability. Under proper conditions, the buffer can retain its effectiveness for an extended period, making it convenient for repeated use in laboratory experiments. -
Question:
Is TAE buffer suitable for RNA electrophoresis?
Answer: TAE buffer is primarily designed for DNA applications but can also be used for RNA electrophoresis. When working with RNA, special care must be taken to avoid degradation, which can be facilitated by using RNase-free reagents and surfaces. TAE buffer maintains a suitable environment for RNA migration, but for specific applications, researchers may choose to use more tailored buffers like MOPS for better results. -
Question:
What is the volume of the TAE buffer bottle?
Answer: This specific product comes in a 500 mL bottle, providing a generous amount of TAE buffer for multiple electrophoresis experiments. This volume is often sufficient for preparing several gels, making it a practical choice for laboratories conducting routine DNA analysis or research. The single bottle format is convenient, ensuring that you have enough buffer readily available without the need for frequent reordering. -
Question:
How does TAE buffer compare to TBE buffer?
Answer: TAE buffer and TBE buffer are both used in DNA electrophoresis, but they have different properties. TAE buffer is gentler on DNA, making it ideal for applications like cloning and visualization, while TBE buffer, containing borate ions, offers better resolution for small DNA fragments due to its higher buffering capacity. The choice between TAE and TBE often depends on the specific requirements of your experiment, such as the size of DNA fragments you're analyzing. -
Question:
What types of DNA can be analyzed using TAE buffer?
Answer: TAE buffer is effective for analyzing a wide variety of DNA types, including genomic DNA, plasmid DNA, and PCR products. It is suitable for both agarose gels and polyacrylamide gels, allowing versatile applications in molecular biology, such as genotyping, cloning, and sequencing. Researchers often use TAE buffer when visualizing DNA with gel electrophoresis, ensuring clear and distinct banding patterns for accurate assessments. -
Question:
Where can I buy Tris Acetate-EDTA Buffer in Solomon Islands?
Answer: You can buy the Tris Acetate-EDTA Buffer 1×, TAE DNA Electrophoresis Buffer, pH 8.0, 500mL bottle from Ubuy. Ubuy is a reliable platform that offers a variety of laboratory supplies, ensuring you have access to high-quality reagents for your experiments. Just visit Ubuy's website, and you can find the TAE buffer along with other necessary laboratory products for your scientific research.
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Features & Benefits
- Ready-to-use reagents save preparation time for experiments.
- Stable acid-base environment ensures biomolecule stability.
- Pre-mixed solutions eliminate dilution hassles.
- Optimal for DNA and RNA separation during electrophoresis.
- Protects nucleic acid samples from degradation.
- Compatible with various gel types and electrophoresis equipment.
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